[Histonet] Demonstration of antigen on cell surface - question?
How would one go about determining if your specific antigen (say for IHC) is located on the cell surface? Right now it seems that the antigen is staining everywhere. (I am running a sheep anti-mouse 'antigen' and using a rabbit anti-sheep biotin conjugated with ABC + DAB for detection. (My negative is normal sheep IgG -- is negative). Is there a way through immuno or other that I can specifically say that the antigen is present on the cell surface? Are there general cell surface markers that I can use along with my primary? Thank you all.
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