Re [Histonet] IHC protocols
Hi,
what we do is:
-HIER with citrate for 15 min, & cool for 20 min
-zymed TRAcP predilute at R Temp for 60 min
-Biotin conjugate anti-Ms IgG for 10 min at R temp
-HRP-streptavidin for 10 min at R temp
-DAB for 5 min at R temp
-counterstain with hematoxylin
i hope this will help
Scott
CSU
Date: Tue, 15 Jun 2004 16:17:00 -0400
From: "Dimaano, Nena"
Subject: [Histonet] IHC protocols
To:
Message-ID:
<20BE9059B6FC2D4B9E8FF2A9E2C4EEBD13F2B9@HOS2KEXCHCL.howost.strykercorp.com>
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Does anyone have a good IHC protocols of TRAcP
antibodies from Zymed?
thanks,
Nena Dimaano
Advanced Technology
Stryker Orthopaedics
325 Corporate Drive
Mahwah, NJ 07430
tel: 201-831-5338
fax: 201-831-6224
email: Nena.Dimaano@stryker.com
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