Re: [Histonet] cryosections problem

From:Gareth Davis

I have cut brain sections from mice and rats, and I have found that floating the cryosections in cold buffer (PBS or Sorenson's) is a great help.  Once the sections hit the PBS they spread and can be floated onto a slide.  I have tried to cut brain sections and immediately attach them to slides, and it never worked.  I usually got air bubbles or wrinkles in the sections.
Hope this helps,
Gareth Davis

Inga Hansson  wrote:
Hi everyone!

Iīm trying to section PFA-fixed and sucrose protected mouse brain.
The sections curl and break when I try to put them on the glass. I
donīt know what temperature to use; is -23 too low? Should I set the
same temperature on the knife as the specimen? Cold or warm slides?

Any suggestions are gratefully appreciated!!



Inga Hansson
dept. neuroscience, div. neurobiology
PO Box 587
Biomedical Centre
Husargatan 3
S-751 23

fax: +46-18-559017

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