RE: [Histonet] phosphorylation state-specific antibodies (PSSA's)

From:"Sennello, Gina"

The brief explanation is ------ they are antibodies used in the study of
protein phosphorylation.  They target phosphorylation state-specific sites
in various kinase cascades.  They are being looked at in cancer research for
possible Biomarker indications and to understand the mechanism of signal
transduction pathways in different diseases. Until recently, they were not
used for IHC, mostly IP and Western blots.  There is a good article in the
American Journal of Pathology, Vol. 163, No 5, November 2003. The Cell
Signaling catalogue is also very good.

So far getting xenograft tumor tissue to stain has been a challenge.  Human
tumors stain pretty well as do cell pellets. If anyone is having success, I
would really like to talk with you.


Gina Sennello
Senior Associate Scientist

2860 Wilderness Place
Boulder, CO

phone 303-546-7739
fax      303-444-0672

-----Original Message-----
[]On Behalf Of Weems,
Sent: Wednesday, June 23, 2004 4:06 PM
To: Patsy Ruegg; Histonet@Pathology. Swmed. Edu
Cc: Ihcrg@Yahoogroups. Com
Subject: RE: [Histonet] phosphorylated antibodies

What are these things?

-----Original Message-----
[]On Behalf Of Patsy
Sent: Wednesday, June 23, 2004 4:35 PM
To: Histonet@Pathology. Swmed. Edu
Cc: Ihcrg@Yahoogroups. Com
Subject: [Histonet] phosphorylated antibodies

Is anyone working with phosphorylated antibodies?  Please share your
experiences with us if you are.
Thank you,

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