Hi Susie,This works with breast and colons that are grossed in fresh at 7 pm for completion the next morning. I don't recall who first told us about this, but I think we heard on Histonet. It has worked well for us for several years. In addition, we still must squeeze the tissue between paper towels to help dehydrate it when the sections are too thick to process well. This processing schedule doesn't hurt this kind of tissue at all.  Joyce-----Original Message-----From: histonet-bounces@lists.utsouthwestern.edu[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of SusieWarwickSent: Monday, June 21, 2004 11:42 PMTo: histonet@lists.utsouthwestern.eduSubject: [Histonet] alcohol to xylene and back to alcohol again!?I am a junior tech, slowly working my way through all the training.mostly I'm at a microtome all day.Anyway I've noticed that our processing schedule also has the backwards alcohol step (Exploding skin samples thread)iealcoholalcoholxylenealcohol <------xylenexylenewaxwaxNow I dont have as much experiance as my supervisor, but it seems like a really weird processing schedule to me.Does anyone else do this? Why is this step in there? I got told it was to help process fatty tissues?_______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonetConfidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer.Thank you. Saint Josephs Health System, Inc.