[Histonet] silver stains

From:"william hewett"

Hi All,
           I was looking for a replacement for chromium trioxide as the oxidizing step in the Grocott's methanamine silver.Placing the slides in periodic acid (5% aq.)heated for 20seconds on medium power in a microwave was an effective alternative. Microwaving the silver solution also saved time and produced less background.Does anyone use a safe microwave method for GMS stains.
                                                Bill Hewett
                                                QHPS  Lab.
                                                Townsville Aust.
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