[Histonet] p38 immunochemistry


I have recently done some immunohistochemical staining with the p38 alpha
antibody.  The antigen unmasking step in the procedure instucts you to soak
the slides in sodium citrate for 1 minute at high temp. (90C) and for 9 min
at medium temp.(60C).  WHen doing this the bones on my slides seemed to
come off.  Does anyone have a suggestion to help this situation?  I need a
way to antigen unmask without having such a bad affect on my slides.

Thanks, Jessica

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