[Histonet] cryosections problem
Iīm trying to section PFA-fixed and sucrose protected mouse brain.
The sections curl and break when I try to put them on the glass. I
donīt know what temperature to use; is -23 too low? Should I set the
same temperature on the knife as the specimen? Cold or warm slides?
Any suggestions are gratefully appreciated!!
dept. neuroscience, div. neurobiology
PO Box 587
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