[Histonet] RE: Tie-2 antibody
We have good experience with the Chemicon rabbit anti-Tie-2 antibody AB3124. Dilution 1:1000 (= 0.2 ug/ml) overnight 4C. Detection with PowerVision-GAR/AP (ImmunoVision) and visualization with Permanent Red (DakoCyto).
Chris van der Loos, PhD
Dept. of Pathology
Academic Medical Center
Amsterdam - The Netherlands
----- Original Message -----
>From K Minhas
Date Tue, 22 Jun 2004 16:12:20 -0400
Subject [Histonet] Tie-2 antibody,
Does anyone have experience with this antibody for Westerns or IHC.
We have tried this antibody from several different sources and just
can't seem to get it to work well. For westerns, only the tissue lysates
from lung show some expression.
Any suggestions would be greatly appreciated, especially for IHC.
Histonet mailing list
<< Previous Message | Next Message >>