[Histonet] RE: [Microscopy] viaWWW: Lynx processor

From:"Flores, Teresa"

Jean, our EM Lab has two Lynx processors and even though they are the most
economical really my first choice would be the Sakura EM Processor (costly)
as Sakura has an enclosed processing system where the solutions go into the
tissues in basket and are not processed like the "old spider auto
technicons" such as the Lynx processor. One drawback the Sakura has or maybe
they have corrected by now is the vapors from the osmium are strong so you
would have to purchase a hood or work with it under a hood, which by what
you are telling me you do already. Our lab has had the same experience with
the long 7 hr processing schedule we have for all tissues except needle bx
of kidney, liver and pancreas which we process in 2 hrs, polymerize in 1and
a half hour and can section HRLM (this is mainly for stat cases
transplants)in which the solutions evaporate when processed overnight, but
as long as it is in spurr epoxy when we come in, it is ok as we feel the
other solutions have processed during the night and we begin our processers
at 5pm when processing overnight.
Teresa Flores
New Orleans, LA

-----Original Message-----
From: jean-ross@uiowa.edu [mailto:jean-ross@uiowa.edu] 
Sent: Monday, June 21, 2004 4:44 PM
To: microscopy@microscopy.com
Subject: [Microscopy] viaWWW: Lynx processor

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21, 2004 at 08:58:37

Email: jean-ross@uiowa.edu 
Name: Jean Ross

Organization: Central Microscopy Research Facility, Univ. of Iowa

Title-Subject: [Microscopy] [Filtered] Lynx processor 

Question: Hello Everyone,

I would like the input of anyone who is a user of the Lynx EM processor.  We
have been having some mixed results in TEM processing of various tissues,
resulting in soft blocks.  

I have observed that the dehydrants are evaporating so that by the time the
tissues reach those stations they are half their original volume.  We have
the unit in a fume hood.  How do others handle possible evaporation and
water uptake issues?  We generally follow our normal protocols that we use
for hand processing.  Any tips or suggestions would be welcome.  

Jean Ross 

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