[Histonet] Blocking in the CSA protocol
Gayle Callis wrote: I have also heard the DAKO avidin/biotin kit
is made up with stronger concentration of avidin in order to accomodate
their CSA (tyramide amplification kit) use.
Kim et. al., An Improved Protocol of Biotinylated Tyramine-based
Immunohistochemistry Minimizing Nonspecific Background Staining
J. Histochem. Cytochem., Jan 2003; 51: 129 - 132. They use 8% Tryptone
Casein Peptone (TCP) and a distilled water/Tween wash to reduce
background staining with their first incubation of SA-HRP. Interesting
read. Haven't tried it yet.
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri 64110
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