Re: [Histonet] fish decaling

From:Gayle Callis

Because of the picric acid in Bouins, most of my fish guru contacts now use
Davidsons with equally good results.  Davidson's has acetic acid and
formalin but does NOT have picric acid. A very nice tradeoff, plus
Davidsons is a popular eye fixative. 

>We usually  fix the fish with Bouin, that also has a little decalcification
>function for a few days, and when we are cutting sometimes is necessary
put the
>parafinn block on HNO3 for +-1h

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology 
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

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