Re: [Histonet] fish decaling
A good start is to fix the fish with Davidson's fixative that contains
acetic acid. Many fish technology people over here use this routinely.
Formic acid can be used to finish the job, you can make up in house formic
acid at 10% concentration which should decalcify more rapidly than buffered
formic acid (~4.5%). You could surface decalcify but it goes through
knives like crazy and tears up soft tissues.
If you do the whole fish, extend processing to allow for good dehydration,
clearing and infiltration. Filleting the fish might tear up inner fine
bones too much. I would assume the fish would decalcify overnight, rinse
for an hour or so and process. We prefer to endpoint test and these days
use a weight loss/weight gain method, simple and easy to do and gets rid of
At 01:11 PM 6/2/2004 -0700, you wrote:
> Calling all histotechs....HELP!
> I am trying to decal a small fish. The size of this fish is 2.5 to
> 3.0cm. Can anyone tell me how long to decal the fish and could I
> surface decal while cutting?
> The idea is to get a full represention of the bones. Would filleting
> the fish firt be better?
> Thanks for the help.
> Carolyn [emsmile.gif] [emrose.gif] Leger
> Seattle Cancer Care Alliance, Seattle, WA
> Watch the online reality show Mixed Messages with a friend and
> enter to win a trip to NY
> 1. http://g.msn.com/8HMBENUS/2752??PS=47575
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