Re: [Histonet] Lymphocytes antibodies - Fixed tissue

From:"Marceau, Gabriel (IAF)(LAVAL)"

Sorry I forgot to specify that I want to detect mice lymphocytes on cryostat
slices of fixed brains and spinal cords.

Gabriel
INRS-Institut Armand-Frappier

----------
      	
From: 	Marceau, Gabriel (IAF)(LAVAL)
[SMTP:Gabriel.Marceau@inrs-iaf.uquebec.ca]  
To: 	'histonet@lists.utsouthwestern.edu' 
Cc: 	 
Subject: 	[Histonet] Lymphocytes antibodies - Fixed tissue 
Sent: 	6/8/2004 10:52 AM 
	Importance: 	Normal 

I Hi everyone,

I am looking for antibodies against lymphocytes (CD3 or other) that will
work on fixed tissue. Most of those comercially available are good for
cytometry but none is marked as usable on paraformaldehyde fixed tissue. If
someone tried one that is not specified for fixed tissue and it worked
please let me know. If not is there a product out there that is said to be
working in these conditions?

Gabriel
INRS-Institut Armand-Frappier

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