Re: [Histonet] IHC negative protocols

From:"Joe Nocito"

I personally think most of the CAP questions need to be re-written. By the
letter of the law, yes, you should have all those negative protocols. In
reality, we just run a mouse and/or rabbit negative per block. What CAP
doesn't realize is that in today's world, someone has to pay for all those
reagents put on the negative slides and you know the insurance companies
won't. (Of course the opinion of this author does not reflect the opinion of
his employer)

Histology Manager
Pathology Reference Lab
San Antonio, TX
----- Original Message ----- 
From: "Dawn Cowie" 
To: "histonet" 
Sent: Wednesday, June 16, 2004 3:21 PM
Subject: [Histonet] IHC negative protocols

> hello netters,
> I have a question regarding negative protocols for IHC. CAP states that
you must use 1 negative control for each species of antibody, per each
pretreatment, per block. We have quite a large menu of antibodies that call
for different pretreatments plus the fact that some are rabbit, some mouse.
In order to comply with CAP we have 16 different negative protocols. From
different people that I have talked to, not too many are following this to
the letter. Is anyone else doing this? Is there an easier way? Are we taking
this too literally? Are we making this more difficult than we need to? Is
this too many questions? Any thoughts, ideas or suggestions would be greatly
> Thanks in advance
> Dawn Cowie HT
> Pensacola Path
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