Re: [Histonet] IHC negative protocols


I've been out of the CAP loop for awhile, but in my protocols for each 
antibody, I include the use of a negative immunogen control and what to 
apply to it.  The negative control is treated just like the rest of the 
slides for that antibody except for the primary antibody.  I don't have 
separate negative control protocols - is that a CAP requirement??


Dawn Cowie 
Sent by:
06/16/2004 03:21 PM

        To:     histonet 
        Subject:        [Histonet] IHC negative protocols

hello netters,

I have a question regarding negative protocols for IHC. CAP states that 
you must use 1 negative control for each species of antibody, per each 
pretreatment, per block. We have quite a large menu of antibodies that 
call for different pretreatments plus the fact that some are rabbit, some 
mouse. In order to comply with CAP we have 16 different negative 
protocols. From different people that I have talked to, not too many are 
following this to the letter. Is anyone else doing this? Is there an 
easier way? Are we taking this too literally? Are we making this more 
difficult than we need to? Is this too many questions? Any thoughts, ideas 
or suggestions would be greatly appreciated.

Thanks in advance

Dawn Cowie HT

Pensacola Path
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