Re: [Histonet] Embedding previously frozen brain

From:Gayle Callis

I don't think it will be entirely free of artifact but we have done
successful thawing/fixation then processing as described. 

To thaw, just drop the frozen brain into formalin, it will thaw while
fixing, change NBF at least once to get rid of OCT and continue to let
brain fix, then process as usual.  

At 11:21 AM 6/10/2004 -0700, you wrote:
>Hello Netters,
>I have a question that came from an investigator at my institute:
>Can mouse brain that has been snap frozen in liquid nitrogen (stored 
>at -80 C degrees) be thawed and embedded in OCT for cryosectioning 
>without causing excessive damage?  How can this be done?
>I appreciate all of your answers and thank you all in advance.
>Take care,
>Jo Dee
>Jo Dee Fish
>Research Technologist III
>Gladstone Institute of Cardiovascular Disease
>Telephone: (415) 695-3720
>Fax: (415) 285-5632
>Mailing address:
>Gladstone Institutes
>P.O. Box 419100
>San Francisco, CA 94141-9100
>Histonet mailing list
Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology 
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

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