Re: [Histonet] Cresyl echt violet acetate vs Cresyl violet acetate

From:Gayle Callis

We buy cresyl violet acetate from Sigma, can buy it other places also -
ACROS via Fisher.  No differences, just nomenclature English versus German.
 Look for the CI (color index) number, if it is the same, it is certified
by Biological Stain Commission, and also look at formula aka molecular
weight.  These are clues to sameness! 

Luxol Fast blue is classic Kluver Barrera, found in AFIP manual, and just
about every histotechnology textbook.  We don't bother to counterstain most
of the time, but it can be done with Nuclear Fast Red, PAS, or cresyl
violet if needed.  

 

At 11:10 AM 6/3/2004 -0500, you wrote:
>Hello histonetters,
>
> 
>
>My question is: Is there a difference in cresyl echt violet acetate and
>cresyl violet acetate ? And what do you out there use for the Luxol Fast
>Blue procedure ?  I have cresyl violet acetate but can't seem to find cresyl
>echt violet acetate anywhere to order it. Please help!
>
> 
>
>Thank You,
>
> 
>
>Ruth Cazares
>
> 
>
>
>
>
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology 
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)



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