RE: [Histonet] quantitative iron testing/processor solutions

From:"Patsy Ruegg"

we always used eosin for this purpose but if you do any epi-fluorescence
eosin does fluores

-----Original Message-----
[]On Behalf Of Hoops,
Sent: Friday, June 18, 2004 8:07 AM
To: ''
Subject: [Histonet] quantitative iron testing/processor solutions

Our lab recently had a case sent out for quantitative iron analysis that
yielded abnormal results.  The pathologists requested we submit a sample of
the methylene blue dye we had been using to color our processing alcohols.
Based upon the sample assays, the methylene blue was the cause of the
elevated metal levels. This raises several questions --
Do other laboratories utilize methylene blue coloring in their processor
If so, have you ever experienced a similar situation with heavy metal
Does anyone use Fast Green (Light Green) for a coloring agent in the
alcohols of their processor?  If so, does it interfere with testing?
Does anyone know of an agent we can use to color our alcohols (tissues) that
does not hinder quantitative iron and/or heavy metal analysis?
Thanks for your help

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