RE: [Histonet] burn artifact

From:"Bill Sinai"


Describe burn artefact!

We had a problem with endometrial curettings but only from one clinician.
The glands of the mucosa appeared to be "burnt" with amorphous nuclear
staining and some eosinophilia only in the mucosa.  We tried fresh fixative,
a new batch of fixative even a new supplier, all to no avail.  All other
specimens were fixed well with no artefact seen in any of the fixatives.
The processing of all other materials appeared OK when together in the
processor.  One of our pathologists discussed the problem with the clinician
and it turned out he would scape several times on to dry gauze then place
the gauze and specimen into fixative.  When we asked him to scape onto
saline dampened gauze the problem all but disappeared.  We concluded that it
must have been a drying artefact.  We still get some pieces showing this
phenomenon but much less often.

All the best from down under.
Bill Sinai
Laboratory Manager
Tissue Pathology, ICPMR
Westmead NSW 2145
Ph 02 9845 7774

-----Original Message-----
[]On Behalf Of MICHELE M
Sent: Thursday, 17 June 2004 8:28 AM
Subject: [Histonet] burn artifact

Hi All,

One of our Pathologists is complaining about a "burn artifact" on his GYN
slides. We lowered the temp of our paraffin baths to 58 degrees in hopes
that they would look better, but he still is seeing this artifact. We
process the tissues for about 5 hours total.  Anyone have any ideas about
what may be causing this?



Do you Yahoo!?
New and Improved Yahoo! Mail - Send 10MB messages!
Histonet mailing list


This electronic message and any attachments may be confidential.  If you
are not the intended recipient of this message would you please delete the
message and any attachments and advise the sender. Western Sydney
Area Health Services (WSAHS) uses virus scanning software but excludes 
any liability for viruses contained in any email or attachment.

This email may contain privileged and confidential information intended
only for the use of the addressees named above. If you are not the
intended recipient of this email, you are hereby notified that any use,
dissemination, distribution, or reproduction of this email is prohibited. If
you have received this email in error, please notify WSAHS

Any views expressed in this email are those of the individual sender 
except where the sender expressly and with authority states them 
to be the views of WSAHS.

Histonet mailing list

<< Previous Message | Next Message >>