RE: [Histonet] Was rat skin - now DeCloaker

From:Margaret Blount

Dear Ronald,

Would you mind please sending me details of your replies on the subject of
rat skin disrupting. I am having similar problems with mouse skin and it is
disrupting exactly where I need to be able to see it!



Margaret Blount
Chief Technician
Clinical Biochemistry
University of Cambridge
Addenbrooke's Hospital
Hills Road
CB2 2QR 

-----Original Message-----
[]On Behalf Of Ronald P.
Sent: Monday, June 07, 2004 2:49 PM
To: Histonet
Subject: [Histonet] Was rat skin - now DeCloaker

Thanks to all who responded to my problem with the rat skin disrupting. We
will try some of the tips with our next batch of staining. Someone had
recommended using Biocare's DeCloaker and Reveal solution. This raises the
next question - is there a definite advantage of using the DeCloaker over
the rice steamer. Certainly there is a big cost difference, $1200 vs. $25.
Is it worth the extra money? We have a lot of IHC work with a variety of
antibodies; Ki67, cytokeratins, cyclins, etc. ahead of us. I do have some
money to spend, so if the collective wisdom of Histoneters suggest that it
is worth the cost I will probably purchase one.

Ronald P. Wilson
Department of Comparative Medicine, HO54
Penn State University College of Medicine
M. S. Hershey Medical Center
500 University Drive
Hershey, PA 17033
phone:  717-531-8460
fax:       717-531-5001

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