RE: [Histonet] TB Exposure

From:"Horn, Hazel V"

We don't even have freezing propellants.....  We previously had used
them only on frozens but now we don't use them at all.

Hazel Horn, HT/HTL (ASCP)
Histology Supervisor
Arkansas Children's Hospital

Phone - 501.364.4240
Fax - 501.364.3912 

-----Original Message-----
[] On Behalf Of Jennifer
Sent: Wednesday, June 02, 2004 11:13 PM
To: Bauer, Karen
Cc: Histonet (E-mail)
Subject: Re: [Histonet] TB Exposure

An important point that has not been mentioned.  Freezing propellants,
such as Cyto-cool, should never be used with unfixed tissue.  If you
refer to NCCLS Document M29-T
    "Frozen sections done on unfixed tissue pose a high risk because
accidents are common.  Freezing does not inactivate infectious agents.
Freezing propellants under pressure should not be used for frozen
sections as they may cause spattering of droplets of infectious
Jennifer MacDonald
Mt. San Antonio College
 If we are
doing a frozen on TB infected lung tissue, with no hood or ventilation
drawing the air away from us, spraying Cyto-Cool all over it (probably
causing the bacteria to scatter all over), then why do we get fitted for
special masks to protect ourselves?  We do wear our masks if we know a
patient is infected with TB, but do not routinely wear them for every
lung case.  And, if we are only exposed by being with the patient, then
we should never have to wear our masks. 

How are others dealing with this?

Thanks in advance!!

Karen Bauer HT(ASCP)
Histology Supervisor

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