RE: [Histonet] Embedding previously frozen brain

From:"Antonia Abeyta"

I personally find it easier to mount it directly on OCT from the -80. 
In my experience, if you embed the tissue, the sections are more likely
to curl.


>>> "GUTIERREZ, JUAN"  6/11/2004
7:47:45 AM >>>
The way we thawed specimens at a lab I used to work for, is by putting
the frozen tissue in refrigerated PBS for a few minutes.  We then patted
it dry on a piece of filter paper and proceeded with the OCT embedding. 
Of course that's if you want your tissue completely surrounded by OCT. 
If not, you can just mount it directly in OCT from the -80.

Juan C. Gutierrez, HT(ASCP)
Histology Laboratory Supervisor
Christus Santa Rosa Hospital
333 N. Santa Rosa Ave.
San Antonio, TX  78207

-----Original Message-----
From: Jo Dee Fish [] 
Sent: Thursday, June 10, 2004 1:22 PM
Subject: [Histonet] Embedding previously frozen brain

Hello Netters,
I have a question that came from an investigator at my institute:

Can mouse brain that has been snap frozen in liquid nitrogen (stored 
at -80 C degrees) be thawed and embedded in OCT for cryosectioning 
without causing excessive damage?  How can this be done?

I appreciate all of your answers and thank you all in advance.

Take care,
Jo Dee



Jo Dee Fish
Research Technologist III
Gladstone Institute of Cardiovascular Disease

Telephone: (415) 695-3720
Fax: (415) 285-5632

Mailing address:
Gladstone Institutes
P.O. Box 419100
San Francisco, CA 94141-9100

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