> Has anyone had any success working with CD 52 in paraffin sections?
>  
> We are using the rat anti-human CD52 from Serotec. CD 52 is 
> meant to be a
> cell surface membrane marker, but we are getting nuclear 
> staining. 

Welcome to the club of unsatisfied CD52-users! I have discussed this problem
quite often with other pathologists, as far as I know the results are VERY,
VERY unpredictable. Tonsil as coontrol is okay, but if you use EDTA
decalcified bone marrow trephines (what you normally want to do in a case of
CLL, I presume), the things are getting even worse, with bright staining of
almost all nuclei in the BM, excluding only some osteoblasts...

Alexander Nader MD
Path. Institut Hanusch-Krankenhaus

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