[Histonet] blocking endogenous peroxidase when using ABC staining and DAB


Hi,I'm working as a lab technician at UF making using 
immunohistochemistry (ABC method and then DAB, specifically) to 
find out where eNOS is located in sheep placenta tissue. However, 
I seem to be both not getting staining on the endothelial cells 
(which is weird since I'm using eNOS antibody) and getting a lot 
of staining due to endogenous peroxidase activity. I've already 
tried putting the slides in a solution of 1:10 30%hydrogen 
peroxide to methanol for as long 40 min, and it still doesn't seem 
to quench the endogenous peroxidase. Anyone have any suggestions 
as to other methods of quenching it?
"You are a child of the universe, no less than the trees and the 
You have a right to be here and whether or not it is clear to you, 
no doubt the universe is unfolding as it should. Therefore be at 
peace with G-d, whatever you conceive Him to be. And whatever your 
labors and aspirations,in the noisy confusion of life, keep peace 
in your soul.-Max Ehrmann,"Desiderata"

Histonet mailing list

<< Previous Message | Next Message >>