Hi,I'm working as a lab technician at UF making using 
immunohistochemistry (ABC method and then DAB, specifically) to 
find out where eNOS is located in sheep placenta tissue. However, 
I seem to be both not getting staining on the endothelial cells 
(which is weird since I'm using eNOS antibody) and getting a lot 
of staining due to endogenous peroxidase activity. I've already 
tried putting the slides in a solution of 1:10 30%hydrogen 
peroxide to methanol for as long 40 min, and it still doesn't seem 
to quench the endogenous peroxidase. Anyone have any suggestions 
as to other methods of quenching it?
--
SMITH,REBEKAH FELICIA
"You are a child of the universe, no less than the trees and the 
stars
You have a right to be here and whether or not it is clear to you, 
no doubt the universe is unfolding as it should. Therefore be at 
peace with G-d, whatever you conceive Him to be. And whatever your 
labors and aspirations,in the noisy confusion of life, keep peace 
in your soul.-Max Ehrmann,"Desiderata"


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet