[Histonet] Re: Microscopic Bubbles...by the hundred
From: Greg Dobbin
Subject: (Fwd) Re: Microscopic Bubbles...by the hundred
Date sent: Tue, 8 Jun 2004 09:22:12 ADT
From: Greg Dobbin
To: Bruce Abaloz
Subject: Re: [Histonet] Microscopic Bubbles........by the hundreds & thousands
Date sent: Tue, 8 Jun 2004 09:20:47 ADT
This this is just a wild guess, but it sounds like you're up for any
Maybe you need to look to the leaf structure to find the answer to
the bubbles. Maybe the stomas (stomi?) are the source of the
bubbles?? Are you cutting cross-sections of the leaves or planar
(broad surface)? I would think the former would be less apt to have
bubbles if indeed the stomas are the source.
Date sent: Tue, 08 Jun 2004 11:49:03 +1000
From: Bruce Abaloz
Subject: [Histonet] Microscopic Bubbles........by the hundreds & thousands
> Dear Histonetters,
> We have done the Oil Red O Charukian Method and Oil Red O technique
> from Bancroft & Stevens, 3rd edition - both worked beautifully. Also
> did Toluidine Blue stain combo with the Oil Red O & by itself - all
> stunning results. Did the stains on cryo -sections of a native
> Australian plant to demonstrate that the 'grainy' bits on the
> underside of the leaves were full of Lipids/fats.
> The PROBLEM we have encountered, is - no matter what Aqueous
> Mountant we try , our cryosections are covered in microscopic air
> bubbles (have tried to date: BDH/ Aquamount; DAKO /Faramount &
> Glycergel & Ultramount.....even plain ol' Glycerol, (coverslipping
> from DH20/Deionized H20/Tap H2O/Hot H2O/air dried - no H20).......
> ALL resulted with same problem.
> Can someone PLEASE advise what we are doing wrong (???) OR suggest a
> possible answer as we need these results to be photographed ASAP.
> Cheers & THANKS in advance,
> Bruce in Melbourne/OZ
> BRUCE ABALOZ PH:61383446282
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