----- Original Message ----- 
From: 
To: 
Sent: Monday, June 07, 2004 10:01 AM
Subject: Histonet Digest, Vol 7, Issue 10


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> Today's Topics:
>
>    1. fibrin stain (Cathy Mayton)
>    2. free leftover control blocks (Histologia@aol.com)
>    3. cryosectioning intervertebral disc  (anclg)
>    4. Rho B (Inga Hansson)
>    5. RE: (no subject) (Walzer Susan)
>    6. Re: markers (tbergeron@criver.com)
>    7. Was rat skin - now DeCloaker (Ronald P. Wilson)
>    8. Supplies needed/protocols for (T. Montara)
>    9. RE: looking for antibodys (r v weimer)
>   10. Re: Supplies needed/protocols for (Gayle Callis)
>   11. Reference for the use of Bromsulphalein or BSP for celaning
>       of glassware (Smallwood, Lorraine)
>   12. RE: Capsase (mark.lewis@thermo.com)
>   13. TUNEL kit, further info (Katy Whalley)
>   14. Re: RE: Capsase (Geoff McAuliffe)
>   15. RE: Was rat skin - now DeCloaker (Margaret Blount)
>   16. Melanoma Cocktail (Dawson, Glen)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Sun, 6 Jun 2004 10:11:43 -0700
> From: "Cathy Mayton" 
> Subject: [Histonet] fibrin stain
> To: "Histonet" 
> Message-ID: <005101c44be9$58aaaca0$f9683442@selfuggnbwd3wt>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Fellow Histonetters,
>
> We have been using Carstairs' fibrin and platelet stain.  It is in
Sheehan's book.  Of all my histo books this is the only one it is in.
>
> Hope this helps,
> Cathy
> ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
> "Quality Histology with a Personal Touch"
> A GLP Compliant Laboratory
>
> Cathy A. Mayton
> Wasatch Histo Consultants, Inc.
> 775-625-4425
> www.wasatchhisto.com
> ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
>
> ------------------------------
>
> Message: 2
> Date: Sun, 6 Jun 2004 18:49:48 EDT
> From: Histologia@aol.com
> Subject: [Histonet] free leftover control blocks
> To: histonet@lists.utsouthwestern.edu
> Message-ID: <65.2b5ad5f1.2df4f98c@aol.com>
> Content-Type: text/plain; charset="US-ASCII"
>
> Free leftover control blocks. Just pay mailing/ costs.Processed for IHC
and
> special stain requirements.Otherwise we will destroy them. If interested
please
> email me. Marina at histologia@aol.com.
>
>
> ------------------------------
>
> Message: 3
> Date: Mon, 7 Jun 2004 12:25:11 +0100
> From: "anclg" 
> Subject: [Histonet] cryosectioning intervertebral disc
> To: 
> Message-ID: <000001c44c82$1aade540$8f6ede89@hist143>
> Content-Type: text/plain; charset="us-ascii"
>
> Hi everyone,
>     I`m posting this on behalf of a collegue who wondered if I could
>     suggest anything to help, but I have had limited success myself
> despite
>     trying frozen, wax embedding and resin embedding this tisse. Can
> anyone
>     help please!! Thanking you all in anticipation of a miracle method.
>     Chris.
>
>   :-  The volume of the disc tissue (mainly annulus) is approx 1cm^3
> snap
>     frozen is isopentane.  So far we have only been able to take
> sections
>     of the
>     annulus perpendicular to the spinal axis (ie transverse sections).
> I
>     would
>     ideally like to take sections of unfixed tissue not only in the
>     transverse
>     plane but also radial sections and sections parallel to layers.
>     The problems we are experiencing are:
>     *mounting sections to slide - we are currently using APES coated
> slides
>     and have
>     tried various slice thicknesses from 10 - 40 microns and heating the
>
>     slides to
>     increase the temperature differential
>
>     *mounting sections flat - The sections are normally folded or
> ruffled
>
>     *tears in sections - we have increased the temperature of the table
>     from 15degC
>     to 20 degC
>
>     *Flaking when sectioning parallel to layers - no suggestions for
> this
>     one yet!
>
>
>
>
> ------------------------------
>
> Message: 4
> Date: Mon, 7 Jun 2004 13:14:28 +0200
> From: Inga Hansson 
> Subject: [Histonet] Rho B
> To: histonet@lists.utsouthwestern.edu
> Message-ID: 
> Content-Type: text/plain; charset="us-ascii" ; format="flowed"
>
> Hi everyone!
>
> Does anyone know of an anti body against Rho B (than the one from
> Santa Cruz) that would work on mouse FFPE tissue? It gives lots and
> lots of background!!
> Thanks in advance!
>
> Inga
> -- 
>
>
> Inga Hansson
> dept. neuroscience, div. neurobiology
> PO Box 587
> Biomedical Centre
> Husargatan 3
> S-751 23
> Uppsala
> SWEDEN
>
> phone:+46-18-4714384
> fax: +46-18-559017
>
>
>
> ------------------------------
>
> Message: 5
> Date: Mon, 7 Jun 2004 07:49:19 -0500
> From: Walzer Susan 
> Subject: RE: [Histonet] (no subject)
> To: 'Diana McCaig' ,
> histonet@lists.utsouthwestern.edu
> Message-ID:
> <492A62ABF11DA240B063BC8278A88A3461107E@orlex03.hca.corpad.net>
> Content-Type: text/plain; charset="iso-8859-1"
>
>
> We do Pascual's modification of Grimelius Stain, you should be able to
find
> procedures online or in a stain manual.
> -----Original Message-----
> From: Diana McCaig [mailto:dmccaig@ckha.on.ca]
> Sent: Friday, June 04, 2004 7:41 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] (no subject)
>
>
> What special stain is recommended to confirm the diagnosis of carcinoid
> tumor on a rectal polyp.   Iff you can help me, can  you provide a
procedure
> as well.  Truly appreciated.
> Diana McCaig, R.T.
> 519-352-6401 (6604)
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 6
> Date: Mon, 7 Jun 2004 07:25:31 -0400
> From: tbergeron@criver.com
> Subject: Re: [Histonet] markers
> To: "Antonia Abeyta" 
> Cc: histonet@lists.utsouthwestern.edu,
> histonet-bounces@lists.utsouthwestern.edu
> Message-ID:
> 
> Content-Type: text/plain; charset=US-ASCII
>
>
>
>
>
> We've been using the PathPen for a while and found that they wash off to
> some extent up to 80% of the time..  We currently have both Surgipath and
> Sakura cassettes in our lab and found this pen washed off both cassettes
> but was more apt to come off the Sakura cassettes than the Surgipath.
>
>       We just received a box of StatMark pens from Statlab and will be
> testing those starting today.
>
> Tracy E. Bergeron, BS, HT(ASCP)
> Histotechician
> Charles River Laboratories
> Wilmington, MA
> 978-658-6000
> x-1229
>
>
> |---------+----------------------------------------->
> |         |           "Antonia Abeyta"              |
> |         |                 |
> |         |           Sent by:                      |
> |         |           histonet-bounces@lists.utsouth|
> |         |           western.edu                   |
> |         |                                         |
> |         |                                         |
> |         |           06/03/2004 04:04 PM           |
> |         |                                         |
> |---------+----------------------------------------->
>
>---------------------------------------------------------------------------
------------------------------------|
>   |
|
>   |       To:       ,
                                  |
>   |       cc:
|
>   |       Subject:  Re: [Histonet] markers
|
>
>---------------------------------------------------------------------------
------------------------------------|
>
>
>
>
> I just recieved a sample PathPen from Pacific Southwest Lab Equipment
> Inc.  I ran it through varying concentrations of EtOH and it didn't come
> off, it's also supposed to be waterproof.  Their phone #: 866-429-0618.
> >From what I understand they are a fairly new company, maybe they would
> be willing to send you a sample.  I haven't used it on cassettes, but it
> might be worth a shot.
>
> Antonia
>
>
> Antonia L. Abeyta
> Health Sciences Tech. III
> Community Environmental Health Program
> University of New Mexico
> Surge Bldg. Room 140
> Albuquerque, NM  87131
> (505) 272-4028
>
> >>> "Vigil, Debbie  (CTMC / CTPA)"  6/3/2004
> 1:48:05 PM >>>
> We have had a sudden problem with our markers.
> All of a sudden the ink is washing off of our cassettes.
> We had that problem a while back and found out it was our cassettes.
> The surface was too smooth.  But we currently use two different
> vendors
> for our cassettes.  We have tried several different markers but the
> same
> thing
> is happening.  Any suggestions would be appreciated.
>
> We have tried secureline, staedtler, and moist mark.
>
> Thank You in advance.
>
> CONFIDENTIALITY NOTICE: This e-mail message, including any attachments,
> is
> for the sole use of the intended recipient(s) and may contain
> information
> that is privileged, confidential and exempt from disclosure under
> applicable
> law. Any unauthorized review, use, disclosure or distribution is
> prohibited.
> If you are not the intended recipient, please contact the sender by
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> e-mail and destroy all copies of the original message.
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
>
>
>
> ------------------------------
>
> Message: 7
> Date: Mon, 7 Jun 2004 09:49:07 -0400
> From: "Ronald P. Wilson" 
> Subject: [Histonet] Was rat skin - now DeCloaker
> To: "Histonet" 
> Message-ID: 
> Content-Type: text/plain; charset="iso-8859-1"
>
> Thanks to all who responded to my problem with the rat skin disrupting. We
> will try some of the tips with our next batch of staining. Someone had
> recommended using Biocare's DeCloaker and Reveal solution. This raises the
> next question - is there a definite advantage of using the DeCloaker over
> the rice steamer. Certainly there is a big cost difference, $1200 vs. $25.
> Is it worth the extra money? We have a lot of IHC work with a variety of
> antibodies; Ki67, cytokeratins, cyclins, etc. ahead of us. I do have some
> money to spend, so if the collective wisdom of Histoneters suggest that it
> is worth the cost I will probably purchase one.
>
> Ronald P. Wilson
> Department of Comparative Medicine, HO54
> Penn State University College of Medicine
> M. S. Hershey Medical Center
> 500 University Drive
> Hershey, PA 17033
> phone:  717-531-8460
> fax:       717-531-5001
> e-mail:  rpw4@psu.edu
>
>
>
>
>
> ------------------------------
>
> Message: 8
> Date: Mon, 7 Jun 2004 09:51:21 -0400
> From: "T. Montara" 
> Subject: [Histonet] Supplies needed/protocols for
> To: 
> Message-ID: <000801c44c96$84e5e200$ae2bf79b@thunderbolt>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Please tell me what supplies I need to begin undecalcified bone
processing. I have addressed the aquisition of knives, knife holder, and
block holder.
>
>     What I need now are;
>     1. Which plastic is best for bone?
>     2. What kind of embedding molds and what sizes do they come in?
>         (We are going to do rat legs)
>     3. What kind of instruments will I need?
> I'm new at this and feeling my way, please HELP!
>                         Thanks
>                              Thelma
>
> ------------------------------
>
> Message: 9
> Date: Mon, 07 Jun 2004 10:11:24 -0400
> From: "r v weimer" 
> Subject: RE: [Histonet] looking for antibodys
> To: int09018@alphahunt.com, histonet@lists.utsouthwestern.edu
> Message-ID: 
> Content-Type: text/plain; format=flowed
>
> >From: "HCS" <int09018@alphahunt.com>
> >To: "Histonet" <histonet@lists.utsouthwestern.edu>
> >Subject: [Histonet] looking for antibodys
> >Date: Thu, 3 Jun 2004 14:20:17 -0700
> >
> >Hi,  does anyone have a source for "murine CD11b and/or
> TNF-alpha"?
> >
> >Also, the procedure for either murine CD11b and/or TNF-alpha?
> >
> >Thanks
> >LeRoy Brown HT(ASCP)HTL
> >HCS
> >www.histocs.com
> >1-360-966-7300
>
> MSRS Primary Antibody Database: Access
>
> The MSRS Primary Antibody Databases online has 200,000+ from 550+
companies.
>   Please read the FAQ's page for information regarding
> searching/troubleshooting.
>
> Temporary  ACCESS CODES are:
>
> USER NAME:     tempor
>
> USER ID:           msrs0604abx
>
> Please remember that access codes are case sensitive - use only lower
case.
>
> >_______________________________________________
> >Histonet mailing list
> >Histonet@lists.utsouthwestern.edu
> >http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _________________________________________________________________
> Looking to buy a house? Get informed with the Home Buying Guide from MSN
> House & Home. http://coldwellbanker.msn.com/
>
>
>
>
> ------------------------------
>
> Message: 10
> Date: Mon, 07 Jun 2004 08:19:39 -0600
> From: Gayle Callis 
> Subject: Re: [Histonet] Supplies needed/protocols for
> To: "T. Montara" ,
> Histonet@lists.utsouthwestern.edu
> Message-ID: <3.0.6.32.20040607081939.00c0b810@gemini.msu.montana.edu>
> Content-Type: text/plain; charset="us-ascii"
>
> Methyl methacrylate is superior - first and foremost - you need fume hood
> and careful handling to avoid toxic fumes.
>
> YOu will need a POWERFUL, hopefully automated microtome, Leica 2155 or
> 2165, Tungsten carbide knives (more than one from Delaware Diamond Knives,
> d profile in a universal type knife holder that can be used for c profile
> steel knives also. A vacuum system or pump with vacuum desiccators (must
> work in a fume hood vented to outside is best, fumes are very toxic to
> Central nervous system), refrigerator, final block should be shaped with a
> saw or grinding to fit into block holder, MMA blocks are very hard and can
> be cut to fit into a screw down block holder. An oven to cure to final
> block )after polymerization.
>
> You cannot use conventional molds very easily, as evaporation is too great
> and this creates problems with plastic. You need to place samples inside a
> "mold", unusally invented by you! Round polypropylene containers to fit
> legs into with a screw on lid. Containers that hold samples in formalin
can
> be used as they have lids that with screw or snap on,  plus you may need
to
> do prepolymerized layers - a nice way to have flat bottom blocks.
>
> A waterbath for polymerization for even heating at 37C for polymerization,
> doing this in an oven will cause fumes and bubble formation.
>
> I suggest you start reading about methyl methacrylate, Journal of
> Histotechnology has had some superb publications over the last 15 years,
by
> Sterchi, Mayton(Sanderson), Chappard, Hand, Jenkins, and others.
>
> Lots of patience is needed to work with MMA - there are some workshops
this
> year at National Society of Histotechnology, Toronto Canada, Ruth
> Yaskovitch and another lady are presenting a very good one on just what
you
> need.  It would be a good investment to take a workshop like this, you get
> so SEE and HEAR about MMA methods which helps you avoid problems.
>
> Good luck
>
>
>
>  At 09:51 AM 6/7/2004 -0400, you wrote:
> >Please tell me what supplies I need to begin undecalcified bone
> processing. I have addressed the aquisition of knives, knife holder, and
> block holder.
> >
> >    What I need now are;
> >    1. Which plastic is best for bone?
> >    2. What kind of embedding molds and what sizes do they come in?
> >        (We are going to do rat legs)
> >    3. What kind of instruments will I need?
> >I'm new at this and feeling my way, please HELP!
> >                        Thanks
> >                             Thelma
> >_______________________________________________
> >Histonet mailing list
> >Histonet@lists.utsouthwestern.edu
> >http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
> >
> Gayle Callis
> MT,HT,HTL(ASCP)
> Research Histopathology Supervisor
> Veterinary Molecular Biology
> Montana State University - Bozeman
> PO Box 173610
> Bozeman MT 59717-3610
> 406 994-6367 (lab with voice mail)
> 406 994-4303 (FAX)
>
>
>
>
>
> ------------------------------
>
> Message: 11
> Date: Mon, 7 Jun 2004 10:19:40 -0400
> From: "Smallwood, Lorraine" 
> Subject: [Histonet] Reference for the use of Bromsulphalein or BSP for
> celaning of glassware
> To: 
> Message-ID:
> <3F0F678D30A99347BCDC815100CFF1C01F1D9D@exchange01.phoebe.com>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Hello Histonetters!  Several months ago someone had posted a reference for
the use of bromsulphalein in cleaning of lab glassware.  I went to the
Histonet archives but I cannot find it.  Could someone please provide this
information again?  I am in need of this information for a reference for the
procedure for an upcoming inspection.
> As always, you guys are the greatest!!
> Thanks!
> Lorraine
>
>
>
> ------------------------------
>
> Message: 12
> Date: Mon, 7 Jun 2004 10:25:37 -0400
> From: mark.lewis@thermo.com
> Subject: [Histonet] RE: Capsase
> Cc: histonet@lists.utsouthwestern.edu,
> histonet-bounces@lists.utsouthwestern.edu
> Message-ID:
> 
>
> Content-Type: text/plain; charset=us-ascii
>
>
> Good Monday Morning everyone,
>
> Could I expect Capsase-3 immunohistochemistry to be effective for
> detecting apoptotic cells in fixed, CONTROL hippocampal sections (in other
> words, tissue that has undergone no experimental manipulation or insult)
>
> Thanks in advance to everyone !
>
> Best regards,
>
> Mark
>
> Mark Lewis
> Product Specialist
> Anatomical Pathology
> Clinical Diagnostics
> Thermo Electron Corporation
> (412) 747-4013
> (412) 788-1097
> E-mail: mark.lewis@thermo.com
>
>
>
>
>
>
> ------------------------------
>
> Message: 13
> Date: Mon, 7 Jun 2004 15:31:46 +0100 (BST)
> From: "Katy Whalley" 
> Subject: [Histonet] TUNEL kit, further info
> To: histonet@lists.utsouthwestern.edu
> Message-ID:
> <3790.194.82.240.48.1086618706.squirrel@jenner.ich.ucl.ac.uk>
> Content-Type: text/plain; charset=iso-8859-1
>
> Hi everyone,
>
> Following further enquires , I'm not sure if I made this clear on the
> protocol I recently posted for using the Roche TUNEL kit. I use the kit
> which labels with FITC, and so my protocol probably doesn't work so well
> if you are using a peroxidase detection system. Hope this clears it up!
>
> Katy
>
>
>
> ------------------------------
>
> Message: 14
> Date: Mon, 07 Jun 2004 10:52:39 -0700
> From: Geoff McAuliffe 
> Subject: Re: [Histonet] RE: Capsase
> To: mark.lewis@thermo.com
> Cc: histonet@lists.utsouthwestern.edu,
> histonet-bounces@lists.utsouthwestern.edu
> Message-ID: <40C4AB67.7060206@umdnj.edu>
> Content-Type: text/plain; format=flowed; charset=ISO-8859-1
>
> Hi Mark:
>
>     Yes, the stain will show apoptosis, but only if it is there. Your
> question is, "Is there apoptosis in the normal  hippocampus?" What
> species (mouse, rat, hampster, elephant)? Adult animals (>8weeks old) or
> ??. What region of the hippocampus? All of these will make a difference.
> Subventricular zone might be a better choice, depending on the above.
>
> Geoff
>
> mark.lewis@thermo.com wrote:
>
> >Good Monday Morning everyone,
> >
> >Could I expect Capsase-3 immunohistochemistry to be effective for
> >detecting apoptotic cells in fixed, CONTROL hippocampal sections (in
other
> >words, tissue that has undergone no experimental manipulation or insult)
> >
> >Thanks in advance to everyone !
> >
> >Best regards,
> >
> >Mark
> >
> >Mark Lewis
> >Product Specialist
> >Anatomical Pathology
> >Clinical Diagnostics
> >Thermo Electron Corporation
> >(412) 747-4013
> >(412) 788-1097
> >E-mail: mark.lewis@thermo.com
> >
> >
> >
> >
> >_______________________________________________
> >Histonet mailing list
> >Histonet@lists.utsouthwestern.edu
> >http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
> >
>
> -- 
> --
> **********************************************
> Geoff McAuliffe, Ph.D.
> Neuroscience and Cell Biology
> Robert Wood Johnson Medical School
> 675 Hoes Lane, Piscataway, NJ 08854
> voice: (732)-235-4583; fax: -4029
> mcauliff@umdnj.edu
> **********************************************
>
>
>
>
>
>
> ------------------------------
>
> Message: 15
> Date: Mon, 7 Jun 2004 16:37:35 +0100
> From: Margaret Blount 
> Subject: RE: [Histonet] Was rat skin - now DeCloaker
> To: "'Ronald P. Wilson'" , Histonet
> 
> Message-ID:
> <6C21D947F7189448AB447C57BF2992B4025F16F5@mius.medlan.cam.ac.uk>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Dear Ronald,
>
> Would you mind please sending me details of your replies on the subject of
> rat skin disrupting. I am having similar problems with mouse skin and it
is
> disrupting exactly where I need to be able to see it!
>
> Thanks
>
> Margaret
>
> Margaret Blount
> Chief Technician
> Clinical Biochemistry
> University of Cambridge
> Addenbrooke's Hospital
> Hills Road
> Cambridge
> CB2 2QR
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Ronald P.
> Wilson
> Sent: Monday, June 07, 2004 2:49 PM
> To: Histonet
> Subject: [Histonet] Was rat skin - now DeCloaker
>
>
> Thanks to all who responded to my problem with the rat skin disrupting. We
> will try some of the tips with our next batch of staining. Someone had
> recommended using Biocare's DeCloaker and Reveal solution. This raises the
> next question - is there a definite advantage of using the DeCloaker over
> the rice steamer. Certainly there is a big cost difference, $1200 vs. $25.
> Is it worth the extra money? We have a lot of IHC work with a variety of
> antibodies; Ki67, cytokeratins, cyclins, etc. ahead of us. I do have some
> money to spend, so if the collective wisdom of Histoneters suggest that it
> is worth the cost I will probably purchase one.
>
> Ronald P. Wilson
> Department of Comparative Medicine, HO54
> Penn State University College of Medicine
> M. S. Hershey Medical Center
> 500 University Drive
> Hershey, PA 17033
> phone:  717-531-8460
> fax:       717-531-5001
> e-mail:  rpw4@psu.edu
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 16
> Date: Mon, 7 Jun 2004 11:35:01 -0500
> From: "Dawson, Glen" 
> Subject: [Histonet] Melanoma Cocktail
> To: histonet@lists.utsouthwestern.edu
> Message-ID: 
> Content-Type: text/plain; charset="iso-8859-1"
>
> All,
>
> I've had a number of questions about my Melanoma Cocktail.  Anyone
> interested can find out anything they want to know at
> http://www.biomedcentral.com/1471-2407/3/15
>
> This is our article printed in an online journal.  I didn't hide any
"trade
> secrets" so my exact procedure is included.
>
> Glen Dawson
>
>
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 7, Issue 10
> ***************************************


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