[Histonet] Microscopic Bubbles........by the hundreds & thousands

From:Bruce Abaloz

Dear Histonetters,

We have  done the Oil Red O Charukian Method and Oil Red O technique 
from Bancroft & Stevens, 3rd edition - both worked beautifully. Also 
did Toluidine Blue stain combo with the Oil Red O & by itself - all 
stunning results. Did the stains on cryo  -sections of a native 
Australian plant to demonstrate that the 'grainy' bits on the 
underside of the leaves were full of Lipids/fats.
  The PROBLEM we have encountered, is -  no matter what Aqueous 
Mountant we try , our cryosections are covered in microscopic air 
bubbles (have tried to date: BDH/ Aquamount; DAKO /Faramount & 
Glycergel & Ultramount.....even plain ol' Glycerol, (coverslipping 
from DH20/Deionized H20/Tap H2O/Hot H2O/air dried - no H20)....... 
ALL resulted with same problem.
Can someone PLEASE advise what we are doing wrong (???) OR suggest a 
possible answer as we need these results to be photographed ASAP. 
Cheers & THANKS in advance,

Bruce in Melbourne/OZ
BRUCE ABALOZ                           PH:61383446282
HISTOLOGIST                            FAX:61383447909
DEPT.of ZOOLOGY                        EMAIL: brucea@unimelb.edu.au

                Nobody can make you feel inferior without your 
permission.  -  Eleanor Roosevelt
                          DANCE LIKE NO-ONE'S WATCHING 
This electronic message and all contents contain information which 
may be
privileged, confidential or otherwise protected from disclosure.The 
information is intended to be for the addressee only. If you are not 
the addressee, any disclosure, copy, distribution or use of the 
contents of this message is prohibited. If you have received this 
electronic message in error, please notify us immediately and destroy 
the original message and all copies.
Histonet mailing list

<< Previous Message | Next Message >>