[Histonet] Ki-67 (MIB1) Immunohistochemistry and neutrophil labeling
From: | david.l.krull@gsk.com |
We recently had a Cyno monkey study which required evaluation of cell
proliferation in formalin fixed, paraffin embedded liver sections. During
the review of the slides we noticed that neutrophils were also positive in
addition to proliferating hepatocytes using DAB as the chromagen. When I
switched to Vector VIP the neutrophils were negative. Has anyone else
observed neutrophil labeling with Ki-67 using DAB? Could the DAB be
reacting with the neutrophils?
David L. Krull
Scientist, Molecular &
Ultrastructural Pathology
GSK Safety Assessment 9.2103
5 Moore Drive
RTP, NC 27709-3398
Phone: 919-315-2457
David.L.Krull@GSK.com
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