[Histonet] IHC negative protocols

From:Dawn Cowie

hello netters,

I have a question regarding negative protocols for IHC. CAP states that you must use 1 negative control for each species of antibody, per each pretreatment, per block. We have quite a large menu of antibodies that call for different pretreatments plus the fact that some are rabbit, some mouse. In order to comply with CAP we have 16 different negative protocols. From different people that I have talked to, not too many are following this to the letter. Is anyone else doing this? Is there an easier way? Are we taking this too literally? Are we making this more difficult than we need to? Is this too many questions? Any thoughts, ideas or suggestions would be greatly appreciated.

Thanks in advance

Dawn Cowie HT

Pensacola Path
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