[Histonet] Gms for Pneumo
In Response to Gayle's Inquiry as to how we do our stain,
We use the GMS procedure in Freida Carson's book, but we use the microwave:
Heat the chromic acid @ 80% power for 50 seconds
Put the slides into the hot chromic and let sit for 10 minutes.
Wash and put in bisulfite for I minute and rinse.
Heat silver (25ml stock methenamine silver, 25ml DI water and 2 ml 5% Borax)
37 seconds on high
Place slides into hot silver and microwave on high for 10 seconds, let sit
for 3-5 minutes checking
Under the microscope after 3 minutes.
Rinse, tone with 0.2% gold, then 2% thiosulfate and counterstain with fast
The fungus comes out very well each time, but the pneumo is very
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