[Histonet] Cresyl violet acetate Biological Stain Commission certified

From:Gayle Callis

Although there is no C.I. number, the Biological Stain Commission has
cresyl echt violet certified for use with Powers and Clark method on NBF
and Bouins fixed brain and spinal cord, available from Sigma. 

For our work to stain Nissl, we use the uncertified and it works fine. 

Interesting thing, when we were setting up the stain, we were handed
crystal violet!  Whoops, not the same thing/dye!  Be sure to check labels.  

 02:22 PM 6/4/2004 -0400, you wrote:
>   "Echt" means genuine in everyday German.  In technical German, "echt"
>means colorfast.  The German word for acetate is "essigester."  A few
>Germans now use the international (Latin) "acetat" for acetate.
>   Because neither cresyl violet acetate nor cresylechtviolet has been
>assigned a C.I. number, they may or may not be the same thing.  Most
>currently produced cresylechtviolet is the acetate of cresyl violet, and
>most currently produced cresyl violet is the acetate rather than the free
>base. However, older bottles of cresylechtviolet may contain the
>hydrochloride of cresyl violet or trimethyl cresylviolet.
>Allen A. Smith, Ph.D.
>Professor of Anatomy
>Barry University
>School of Graduate Medical Sciences
>            Podiatric Medicine and Surgery
>Miami Shores, Florida
>-----Original Message-----
>From: histonet-bounces@lists.utsouthwestern.edu
>[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
>Sent: Friday, June 04, 2004 1:08 PM
>To: Histonet@lists.utsouthwestern.edu
>Subject: [Histonet] Echt means acetate so they should be the same
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Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology 
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

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