I would like to use the Formalin-induced Fluorescence to demonstrate adrenergic nerve fibres in intestinal and urogenital tissues. Does anyone have experience with that and would you have a protocol for that? Can I use paraformaldehyde fixed tissue for the method or does it have to be fresh? I would also like to try it on whole mount preparations of bladder wall.
Any help appreciated
Children's Research Centre
Our Ladies Hospital for Sick CHildren
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