[Histonet] CD 52

From:"Houston, Ronnie"

Has anyone had any success working with CD 52 in paraffin sections?
We are using the rat anti-human CD52 from Serotec. CD 52 is meant to be a
cell surface membrane marker, but we are getting nuclear staining. Serotec
are not able to confirm either way whether this is artifactual or real,
saying that they have no evidence to the contrary that nuclear staining
should not be expected.
Microscopically it looks real enough with lymphocytic staining in tonsils,
and with very clean preparations on positive controls and nothing on
We have only been able to find one reference to CD 52's use in paraffin
sections (J Clin Pathol 1994; 47: 313)
Considering that treatment with CAMPATH therapy is pretty drastic, we are
very hesitant to suggest treatment based on the IHC findings, at present
Any help appreciated

Ronnie Houston
Director of Anatomic Pathology
Bon Secours HealthPartners Laboratories
5801 Bremo Road
Richmond, VA 23226
(804) 2877972
(804) 2877906 - fax




The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed.
It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations.
In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information
contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s).  Should
this communication be received in error, please notify the sender immediately either by response e-mail or by phone at 410-442-3250,
and permanently delete the original e-mail, attachment(s), and any copies. 
Histonet mailing list

<< Previous Message | Next Message >>