staining thick, fixed brain slices

From:Colleen Forster


Those of you who do any type of "museum" work. Do you have and would you 
share a protocol for staining nissl in thick slices of fixed tissue. The 
fixation is given below.

I donit have fresh tissue-I am starting with bodies from mortuary 
science that have been embalmed with formalin/phenol/alcohol.  Brains 
are removed for me after human anatomy is finished with the whole body 

Any help will be greatly appreciated.

COlleen Forster
U of MN

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