preparation of fixed, frozen tissue sections for IHC
I'm going to be sectioning some frozen tissue, which was originallly
perfused with paraformaldehyde, with the intention of using these
sections for immunohistochemical staining. Normally, I would be using
parafin-embedded tissue for IHC; what other steps do I need to take
before adding my antibodies? I'm hoping that since the tissue was
originally perfused, all I need to do is thaw out the sections and
continue with my washing and antibody spotting.
Thanks for your help-
Marcus
::-----------------------------------::
Marcus Andrews, PhD
Dept of Physiology
Medical Sciences Building
University of Toronto
Toronto, Ontario
M5S1A8
CANADA
::-----------------------------------::
<< Previous Message | Next Message >>