preparation of fixed, frozen tissue sections for IHC

From:Marcus Andrews

I'm going to be sectioning some frozen tissue, which was originallly 
perfused with paraformaldehyde, with the intention of using these 
sections for immunohistochemical staining.  Normally, I would be using 
parafin-embedded tissue for IHC;  what other steps do I need to take 
before adding my antibodies?  I'm hoping that since the tissue was 
originally perfused, all I need to do is thaw out the sections and 
continue with my washing and antibody spotting.

Thanks for your help-


   Marcus Andrews, PhD
   Dept of Physiology
   Medical Sciences Building
   University of Toronto
   Toronto, Ontario

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