|From:||yichao wu |
Another question is,since the frozen sections are not as good as paraffin-embeded ones in morphology.I wonder who have successfully acquired RNA for RT-PCR from laser microdissected tissue on paraffin sections?I have tried and tried and failed.The kits I used are "Qiagen Rneasy Mini kit"(I have no "Micro kit" at hand) and one from arctrus and one from Gentra called puroscript.And after Dnase digestion,it seems that I have not acquired any RNA from paraffin embeded sections.The fixation is acetone-methanol(1:1).I wonder if such fixation would destroy RNA? And I wonder if heating sections after paraffin embeding is destroyable? How are other procedures should be followed for microdissection on paraffin embed sections? Pls help me out Thank you very much!
And by the way,do you think disposable blazes are better than steel-based knife when sectioning specimens from renal biopsies?
With my best regards,
Yichao WU, Ph.D candidate
Jinling Hospital,Nanjing University,Nanjing, P.R.China