Depends what tissue (frozen/paraffin and mouse/man) your using!!!
For rat brain paraffin sections we used successfully used : Mouse
monoclonal antibody anti-human-Ki67 from (Novocastra) au 1/700 DAKO
envision (DAB) (ref : NCL-Ki67-MM1) with microwaving in normal citrate
Rabbit anti-human-Ki-67 antigen from Dako and Monoclonal anti-human-Ki-67
from Boehringer/Roche didn't work.
I would suggest getting some small intestine tissue (duo, ileum, jejeunum)
as positive controls. You'll see the labelling only in the crypts of villi.
Molecular Biology Scientist
B.P. 37 Labége Innopole
31676 LABEGE CEDEX FRANCE
tel : (33)561004179 fax :(33)561004001
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