Depends what tissue (frozen/paraffin and mouse/man) your using!!!

For  rat  brain  paraffin  sections  we  used  successfully  used  :  Mouse
monoclonal   antibody  anti-human-Ki67  from  (Novocastra)  au  1/700  DAKO
envision  (DAB)  (ref  :  NCL-Ki67-MM1)  with microwaving in normal citrate
buffer.

Rabbit   anti-human-Ki-67 antigen from Dako and Monoclonal anti-human-Ki-67
from Boehringer/Roche didn't work.

I  would suggest getting some small intestine tissue (duo, ileum, jejeunum)
as positive controls. You'll see the labelling only in the crypts of villi.



Nancy Walker
Molecular Biology Scientist

Sanofi-Synthelbo Research
B.P. 37 Labége Innopole
31676 LABEGE CEDEX FRANCE

nancy.walker@sanofi-synthelabo.com
tel : (33)561004179  fax :(33)561004001