does the spectrum of dab change with the addition of blue hematoxylin

From:Andrew Rabinovich

I am a computer scientist and thus this question may seem trivial. Does
the spectrum of dab change as the blue hematoxylin stain is added to the
sample. In other words, is there a chemical reaction between dab and
hematoxylin once both are applied to the tissue. 
Thanks a lot, 

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