brdu in decalcified bone?

From:"Garlits, John"


I am writing to ask if anybody has experience using BRDU in decalcified bone.  We typically use acid decalcification of formalin-fixed mouse bone.  I had heard that the decalcification could cause problems for subsequent BRDU immunohistochemistry.  Is it necessary to use EDTA instead?


John Garlits, M.S.
Senior Research Technician
Hematology Oncology Division
Experimental Hematology Department
St. Jude Children's Research Hospital
332 N Lauderdale
Memphis, TN 38108
Phone: 901-495-3961
Fax: 901-495-2176

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