|From:||yichao wu |
Thank you very much,Tony!
Then I understand the need of "quick freezing" to -70C.I wonder could I only quick freezing to -20C by isopentane? I mean put isopentane at -20C and frozen specimen by it.
Since I once realized that ice crystals form mostly between -30C to -40C.I wonder if I section immediately,not for preservation the specimen,could I only quick freeze to -20C by isopentane?
The other question is, when I use 30% sucrose to dehydrate the specimen for 10 mins, there are much space between the tubules in the specimen.All the tubules apart from each other. But the tubules themselves maintain very well.The cells are clearly seen. How could I avoid this to happen? The "shrink" phenomenon.
Thank you for your kindness!
>From: Tony Henwood