Re: cryostat functioning?
>From: Greg Dobbin
>To: jason madore
>Subject: Re: cryostat functioning?
>Date: Fri, 30 May 2003 16:36:11 -0300 (ADT)
>It does sound as if the ratchet and paul (sp?) are not functioning
>properly. Perhaps the teeth on the ratchet wheel are worn or the
>spring tension for the paul needs be tightened. I would not be very
>confident in knowing the thickness of your sections at that rate.
>If however for the time being (ie until you have it repaired), you are
>not doing morphometric or sterologic studies; getting a thin section
>(albeit, of unkown thickness) may suffice, as long as it has
>consistent thickness in-of-itself. (I know that sentence is way too
>long, but it has been a long week!) So what I mean is, as long as it
>is thin enough for the pathologist to read the necessary stains-
>he/she will tell you if the sections are inadequate.
>I must also say though, I haven't worked in a human diagnostic
>setting, so if that is your situation, quality assurance rules and regs.
>may necessitate getting it fixed before proceeding any further. DO
>NOT allow my "two cents worth" to be the cause of false
>Have a good weekend.
>Date sent: Fri, 30 May 2003 11:45:27 -0700
>From: jason madore
>Subject: cryostat functioning?
> > Recently got an old leica 1800 cryostat up and running in our lab. After
> > blowing the dust off and cooling it down I started sectioning some
> > tissues. I would have to crank the handle around between 5-10 times
> > it would cut the block between sections. Does this seem normal? How
> > confident can I be that the thickness is what I had set it to? If anyone
> > ever had this problem before I would be gratefull for some advice as I
> > somewhat of a novice at this and there is no histotech in out lab.
> > thanks.
> > jason.
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>Atlantic Veterinary College, U.P.E.I.
>550 University Ave.
>Canada, C1A 4P3
>"A farmer is a person outstanding in their field."
Jason, Sometimes its as simple as the bladeholder or chuck not being tight.
The blade should not move if you try to lift it out with a dissecting pick.
If it moves, try cleaning the holder and retightening. Good luck Tom
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