In my lab we usually use acidified hematoxylin and blue washing in running tap water for 10 minutes. No alcaline nor lithium carbonate solution. It works well

Jos#233# Luis




El dia 05/06/2003 17:20 usted envio el siguiente mensaje:
>Date: 5 de Junio de 2003 17:20:34
>From: Gayle Callis  
>Subject: bluing hematoxylin 
>To: histonet@pathology.swmed.edu
>
> DO NOT use sodium hydroxide!  A drop of ammonium hydroxide OR use gentle
> warm (not hot!) water rinse for a full minute in generally sufficient for
> bluing sections.  We have used saturated lithium carbonate, let crystals
> settle, decant solution off top, dip slides for 30 sec or so until blue,
> rinse WELL for 1 full minute. The latter will not hydrolyze the sections
> off the slides as will strong alkaline solutions (sodium hydroxide or even
> too concentrated ammonium hydroxide).  
> 
> One can also makes Scotts tap water substitute in the laboratory.  
> Gayle Callis
> MT,HT,HTL(ASCP)
> Research Histopathology Supervisor
> Veterinary Molecular Biology - Marsh Lab
> Montana State University - Bozeman
> S. 19th and Lincoln St
> Bozeman MT 59717-3610
> 
> 406 994-6367 (lab with voice mail)
> 406 994-4303 (FAX)
> 
> email: gcallis@montana.edu
> 
>