Re: bluing hematoxylin

From:Jose Luis Palazon Fernandez

In my lab we usually use acidified hematoxylin and blue washing in running tap water for 10 minutes. No alcaline nor lithium carbonate solution. It works well

Jos#233# Luis

El dia 05/06/2003 17:20 usted envio el siguiente mensaje:
>Date: 5 de Junio de 2003 17:20:34
>From: Gayle Callis  
>Subject: bluing hematoxylin 
> DO NOT use sodium hydroxide!  A drop of ammonium hydroxide OR use gentle
> warm (not hot!) water rinse for a full minute in generally sufficient for
> bluing sections.  We have used saturated lithium carbonate, let crystals
> settle, decant solution off top, dip slides for 30 sec or so until blue,
> rinse WELL for 1 full minute. The latter will not hydrolyze the sections
> off the slides as will strong alkaline solutions (sodium hydroxide or even
> too concentrated ammonium hydroxide).  
> One can also makes Scotts tap water substitute in the laboratory.  
> Gayle Callis
> Research Histopathology Supervisor
> Veterinary Molecular Biology - Marsh Lab
> Montana State University - Bozeman
> S. 19th and Lincoln St
> Bozeman MT 59717-3610
> 406 994-6367 (lab with voice mail)
> 406 994-4303 (FAX)
> email:

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