SigmaCat# 387A, is the TRAP we used. We used a cold formalin(4 deg) fixation 48-72 hrs, followed by a cold EDTA Decal. Took forever, but worked great. We tried to minimize room temp or heat anything until processing.
Jacob Bentzon <jacob.bentzon@dadlnet.dk>
06/05/03 09:01 AM
To: HistoNet Server <histonet@pathology.swmed.edu>
cc:
Subject: TRAP stain for FFPE decalcified mouse bone
Dear colleagues
I need a protocol for TRAP stain (not IHC) for formalin-fixed,
paraffin-embedded, decalcified mouse bone tissue. I've looked in the
archives and I've seen that this has been discussed before. Could I ask
someone out there to share their knowledge with me.
Thank you
Jacob Bentzon
KMEB, Odense Universitetshospital,
Denmark