If you put a sealed container of isopentane in a -70#176# 
freezer as it cools off pressure increases inside the 
container and will most likely crack the container.  That 
is why everyone is referring to an open container of 
isopentane.

-- 
Karen Bowden
Staff Research Associate II
University of CA, San Diego
Department of Orthopedics
9500 Gilman Dr. 0630
La Jolla, CA 92093-0630
858-277-4970

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JHoffpa464@aol.com wrote:
> oy!!! does anyone look at the practicable side. ???
> 
> 
> if you put a sealed container in a -70C freezer then snap freeze the 
> tissue. is it safe?
> 
> will it work???