Re: HE stain without Blue reagent

From:Joey Shepherd

Re: HE stain without Blue reagent
Hi Yan
You should be able to blue your sections just by running under tap water, but you can try making your own blueing solution - I use Scott's solution (20 g/l of sodium bicarbonate and 3.5 g/l of magnesium sulphate 7-hydrate). After haeamtoxylin, rinse slides in tap water, then immerse in Scott's for 30s-1min, then rinse in tap water before eosin.
Dr Joey Shepherd
Postdoctoral scientist
Academic Unit of Genetics and Informatics
Division of Genomic Medicine
The University of Sheffield Medical School
M Floor, Royal Hallamshire Hospital
Sheffield S10 2JF

Phone 0114 271-3007
Fax 0114-272-1104

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