Re: GMS stain

From:Sarah Jones

Hi Margaret,
  I have a procedure that always works well and is not difficult.  If you send me a fax number, I would be glad to send it to you.  If you have issues of Laboratory Medicine, it is from Vol. 17, No. 2, Feb. 1986.  It's a microwave stain.  

Sarah Jones HT(ASCP)
Dept. of Vet. Anatomy & Public Health
Histology Lab
Texas A&M University
College Station, TX 77843-4458
phone: 979-845-3177
fax:  979-458-3499

>>> SDSU HISTO  06/10/03 01:39PM >>>
The GMS stain is usually a more difficult stain however lately it has become
even harder.  We have made up all fresh reagents, switched to using an
autostainer with an old kit and a new kit and changed from 1%periodic acid
for 1 hour to using chromic acid.  Some of the fungi stain but not all.
This is also happening with the PAS stain. The collagen in the GMS stains a
nice light gray.  
We use the CBG recycler and add acetic acid to our Propar.  Would it be
possible for this to affect the stains?  We use reverse osmosis water and to
my knowledge the water has always been the same.  When doing the stain I get
a silver precipitate if the process goes to long.  Before when this happened
the fungi were dark black.  Now they are barely stained.
Any suggestions would be appreciated.
Margaret Perry
Lab Tech 
Vet Science Histopath
South Dakota State University 

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