Re: Combined Immunohistochemistry with FISH

From:Gayle Callis

Check out these publications

A. U. Zaidi, H. Enomoto, J. Milbrandt, and K. A. Roth
            Dual Fluorescent In Situ Hybridization and Immunohistochemical
            Detection with Tyramide Signal Amplification
            J. Histochem. Cytochem., October 1, 2000; 48(10): 1369 - 1376. 

Mariėtte P.C. van de Corput, Roeland W. Dirks, Rob P.M. van Gijlswijk,
Erica van Binnendijk, Claudia M.
Hattinger, Roelof A. de Paus, Jim E. Landegent, and Anton K. Raap=20
     Sensitive mRNA Detection by Fluorescence In Situ Hybridization Using
     Peroxidase-labeled Oligodeoxynucleotides and Tyramide Signal
     J. Histochem. Cytochem. 1998 46: 1249-1260.  

Paul E. McKeever 
     Insights About Brain Tumors Gained Through Immunohistochemistry and in
Situ Hybridization of
     Nuclear and Phenotypic Markers
     J. Histochem. Cytochem. 1998 46: 585-594.  

At 04:11 PM 6/3/2003 +0100, you wrote:
>Dear all,
>         Hi, I am currently carrying out my project utilizing
immunohistochemistry on FFPE tissues but I am making an enquiry to see if
anyone has had any experiencing of combining immunohistochemistry and
Fluorescent in situ Hybridization (FISH) on the same (FFPE) tissue slide. I
am not sure even if this can be done but I would be really interested in
hearing from anyone who has maybe attempted this with success. I am
interested to know what detection methods you used for immnuohistochemistry
which still allowed visulisation of the fluorescent probes using FISH.
>Thanks in advance for your help
>Lee Ridley
>Birmingham Women's Hospital
>Reproductive & Child Health
>Academic Unit 3RD Floor
>United Kingdom
>B15 2TG
>Tel: +44 (0)121 6074754
Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
S. 19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367 (lab with voice mail)
406 994-4303 (FAX)



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