RE: merosin and emerin on frozen muscle sections.
freezing protocol will give you disastrous results for any muscle work. Your
tissue will be full of ice-crystal artifact. The only way to freeze skeletal
muscle is to snap-freeze in -160C precooled isopentane in liquid
said that, merosin and emerin immunohistochemistry technique will not as such be
affected, just your morphology. You may get some "rings" aroung the ice crystal
holes inside the fibers. Emerin is a nuclear marker.
Without more details about your procedures and source of reagents I can't
offer any other suggestions.
Regional Histology Operations Manager
Secours HealthPartners Laboratories
Richmond, VA 23226
My work is on frozen muscle. I am having trouble with merosin and emerin
immunostaining on frozen muscle. I wonder if my freezing process is to
My freezing procedure is as follows:
1-We recieve the specimen immediately after the biopsy is
2-We put it in a fridge for 15 min to relax.
3- The section is stuck on a pre-frozen chuck with a dab of
4- It is then immediately placed on the quick freeze shelf (-40C) of the
cryostat for 15 min.
5-Then the specimen is put in the main compartment of the
cryostat (-20C) and left there for
20 min. to accomodate to the temperature.
6- The sections are then cut at 8 microns and placed on
7- The slides are left to dry overnight at room temperature.
By the way we get very good results with Dystrophins and Sarcoglycans,
using this method of freezing.
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