Phil,

Autoclaving your slides should be fine, remember, when people do antigen
retrieval, they use a pressure cooker, and all that is is a small version of
an autoclave. Other things that _might_ work are UV irradiation or heating
in an oven (not too sure how well the glass will stand up to that).

Best regards,

Nick

PS

Today is my last day in this job, so I may be away from Histonet for a
little while.
I'm off to Vision BioSystems as their UK Application Support Specialist on
their new immunostainer, so wish me luck!



-----Original Message-----
From: Phil Bergin [mailto:philip.bergin@microbio.gu.se]
Sent: 10 June 2003 09:57
To: Histonet@pathology.swmed.edu
Subject: Superfrost plus slides


Hi,

 

I was just wondering if anyone knows if autoclaving superfrost slides
will affect the charge/binding properties.  I need some sterile slides
and was wondering the best way to go about it.  I have some
rubber/silicon chambers to add onto slides to make 'chamber slides' for
cell cultures.  Unfirtunately I'll then be stimulating the cells with a
live bacteria, so I can't use strong antibiotics- other people here use
slides from an unopened box and PEST- but I'm not sure that'll be
sterile enough for me........

 

Any advice would be much appreciated,

 

Phil

-----------------------------------------------------------------

 

Philip Bergin

Göteborg University

Department of Medical Microbiology and Immunology

Box 435, SE-405 30 Göteborg, Sweden

Phone: +46-31-3424471

Fax: +46-31-826976

 




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